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Exopolysaccharides produced by rhizobacteria isolated from rhizosphere of different plants
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Abstract: Extra cellular polysaccharides (ESP) are components of bacterial cell wall,which play an important role in soil aggregation, interaction between the bacteria and their environment. In this study a comparison between chemical compositions of exo-polysaccharides produced by some nitrogen fixing bacteria strains (Azotobacter chroococcum, Azospirillum brasilense, Bacillus polymyxa, Rhizobium leguminosarum and Bradyrhizobium japonicum) with that of reference-identified strains was done. The extractable exopolysaccharides were anaylysed using thin layer chromatography (TLC) and high pereformance liquid chromatography (HPLC). The data showed that extractable polysaccharides were more heterogenous in their composition than those homogeneous in others. The sugars polysaccharides of Azotobacter chroococcum consisted of mannitol (80.83%from total sugars), lactose (19.16%) while, Azospirillum consisted of galactose (14.05%); xylose (14.05%); arabinose (35.95%), pyruvic (35.95%). In contrast the polysaccharides of Bacillus polymyxa contained ribose (51.42%), glucose (11.98%) raffinose (11.98%); galactose (6.2%); arabinose (6.) pyruvic (6.2%) xylose (6.2%), rbizobial extractable polysaccharides contained galactose (25%), xylose (25%) arabinose (25%); pyruvic (25%) whereas excreted rhizobium polysaccharides comprised galactose (33.33%) ; arabinose (33.33%) and pyruvic(33.33%).
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Publication year |
2004
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Pages |
185-192
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Availability location |
Egyptian Society of Applied Microbiology
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Availability number |
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Organization Name |
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Country |
Egypt
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City |
Cairo
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Publisher |
Name:
Egyptian Society of Applied Microbiology
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serial title |
Proceeding of the 11th Conference of the Egyptian Society of Applied Microbiology ,Cairo, Egypt
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Department |
Agricultural Microbiology Research
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External authors (outside ARC) |
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Agris Categories |
Agricultural research
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AGROVOC TERMS |
Azospirillum brasilense.
Azotobacter.
Bacillus polymyxa.
Bradyrhizobium japonicum.
Polysaccharides.
Rhizobium leguminosarum.
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Proposed Agrovoc |
soil aggregation;
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Publication Type |
Conference/Workshop
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