Quantitation of Capripox purified viral proteins
Abstract: Sheep pox "SP", goat pox "GP" and lumpy skin disease "LSD" viruses were propagated and titrated individually in Vero or MDBK cell cultures and their titres were 5.6, 5.3 and 5.2 log10 TCID50/ml respectively. The viral proteins of these viruses were estimated by Bradford and Lowery techniques using spectrophotometer. The first technique depends on standard curve while the second technique based on Lowery equation. The quantity of viral proteins were 0.306, 1.440 and 2.074 mg/ml for SPV, GPV and LSDV respectively by using Lowery technique, while in Bradford technique, the viral proteins were 0:287, 1.297 and 1.986 mg/ml for SPV, GPV and LSDV respectively . The results in Lowery technique were more sensitive and accurate than those obtained by Bradford technique.
Publication year 2009
Pages 6-7
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
City كفر الشيخ
serial title المؤتمر العلمي الثالث - لكلية الطب البيطري جامعة كفر الشيخ
Author(s) from ARC
External authors (outside ARC)
    محمد سعيد واصل المعمل المركزي للرقابة على المستحضرات الحيوية البيطرية
    ماجدة محمد سيد المعمل المركزي للرقابة على المستحضرات الحيوية البيطرية
    محمد احمد سعد المعمل المركزي للرقابة على المستحضرات الحيوية البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Capripoxvirus. Microbial proteins.
Publication Type Conference/Workshop
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