A trial for preparation of tissue culture vaccine for Gumboro disease by using local isolate on Bursal gland

Abstract: Thirty serial passages of local IBD virus on chicken bursal cell culture (CB) were prepared and titrated. After every 5 passages the virus was inoculated into ten 21 day-old chicken and observed for 21 days post , infection then challenged with virulent IBD virus. The 20th passage induced complete loss of virus pathogenicity and gave 100% protection and used for preparation of live attenuated CB cell culture IBDV vaccine. The evaluation of the prepared vaccine was carried out for sterility, safety and potency. The potency test was performed by measuring humoral immune response as well as protection percentage against virulent IBDV. The neutralizing antibody titre in sera of chickens vaccinated with tissue culture vaccine was higher than thosevaccinated with other commercial vaccine. The efficacy of the prepared vaccine was estimaled for up to four months.

Publication year 2009
Pages 273-285
Organization Name
City راس سدر
serial title المؤتمر الطبي العلمي لكلية الطب البيطري بمشتهر جامعة بنها
Author(s) from ARC
External authors (outside ARC)
    نادية محمد ابراهيم معهد بحوث الامصال واللقاحات البيطرية
Agris Categories Animal diseases
Publication Type Conference/Workshop
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