Trial for Preparation of Monovalent inactivated Equine InfluenzaVaccine

Abstract: Equine influenza virus (A/equi-2 \Cairo-2\2000, H3N8) with egg passage 6 (EP6) was inactivated by 0.1% formaline (40% formaldehyde) and complete inactivation was achieved after 24 hours at 37C. two forms of monovalent equine influenza vaccine were prepared using different adjuvants, the first with DEAE- Dastran, while the other with Alhydragel and saponin. Both vaccine forms (freeze dried and liquid one) proved to be safe and potent for horses and G. pigs. The mean HI antibody titres in G. Pigs (three weeks post inoculation) were 1408, 1344, while in horses (six weeks post inoculation) were 640, 480 respectively for both vaccines. The antibody titre in horses vaccinated with both vaccine forms were monitored and persisted at protective level for one year. The keeping quality of thelocally prepared EI monvalent inactivated vaccine either freeze- dried, reconstituted in DEAE-Dextran or the liquid one were studied and the shelf validity for both vaccines were found to be stable at 4°C for one year, while the freeze dried one can be kept at -20°C for three years
Publication year 2009
Organization Name
serial title the third scientific conference
Author(s) from ARC
External authors (outside ARC)
    ابراهيم محمد أحمد سليمان معهد بحوث الامصال و اللقاحات
    نهال صالح عبد الرحمن معهد بحوث الأمصال البيطرية
Publication Type Conference/Workshop
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