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Trials For Preparation of Tissue Culture Inactivated Freeze Dried EHV-1 Vaccine
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Abstract: Tissue culture inactivated freeze dried EHV-1 vaccine was successfully prepared from the locally isolated strain, egg passage 3 (EP3) with a titre of 7 log10 EID50/ml after 6th tissue culture passage with a titre of 9 log10 TCID50/ml. the virus was completely inactivated by 0.008 MBEI after 24 hours at 37C. The vaccine was reconstituted in DEAE-Dextran as adjuvant just before inoculation. The vaccine was proved to be safe and immunogenic when tested in pregnant mares and mice. The virus neutralizing antibody titre in the inoculated horses was monitored and persisted with a protective level (30 neutralizing antibody) for 6 months. The keeping quality of the prepared vaccine was studied and its shelf validity was found to be stable at -20°C for two years, at 4°C for one years and in room temperature for six months.
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| Publication year |
2009
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| Organization Name |
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| serial title |
animal health research conference
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| Author(s) from ARC |
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| External authors (outside ARC) |
إيمان محمد محمد عبيد
معهد بحوث الأمصال و اللقاحات البيطرية
ماجدة أنيس قلد
معهد الأمصال و اللقاحات
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| Publication Type |
Conference/Workshop
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