Evaluation of lumpy skin disease virus vaccine using cell-mediated immune parameters.
Abstract: Studies on the acquired immunity of cattle inoculated with tissue culture adapted lumpy skin disease virus through cell mediated immune response and immuno-serological tests revealed that :

1- Titration if tissue culture adapted lumpy skin disease virus proved that the titre of the virus was log10 5.2 TCID 50/ml.

2- Cattle were inoculated with previous tissue culture adapted lumpy skin disease virus by the interadermal route. Each animal inoculated 1/D with 0.5 ml have a titre of log10 5.2 TCID 50/ml.

3- Challenging the acquired immunity in inoculated cattle one month post inoculation. Resulted in complete protection against the virulent LSDV where no local or generalized lesions appeared.

4- Results of the lymphocyte transformation technique (by MTT assay) revealed that lymphocytes increased from the 3rd day in cell and cells with mitogen (Con. A) till the 14th post inoculation. While lymphocytes in the challenged cattle was increased from the 2nd day till the 14th day in cells and cells mitogen (Con.A) post challenge, but in the control ones (non inoculated challenged cattle). It was higher than inoculated challenged cattle.

The results predominated that the cell mediated (by MTT assay) technique considered more accurate, easier and faster than the serological one. On the other hand, there was no great variations between the results obtained with the serological (humoral) and cellular experiments used for detection of the immune status of cattle inoculated with lumpy skin disease virus.
Publication year 1997
Availability location معهد بحوث الامصال و اللقاحات البيطرية
Availability number
Organization Name
Country Egypt
Publisher Name: كلية الطب البيطري- جامعة القاهرة
Author(s) from ARC
External authors (outside ARC)
    أ.د.محمد عبد الحميد شلبي استاذ قسم الفيرولوجيا كلية الطب البيطري جامعة القاهرة
Agris Categories Animal diseases
AGROVOC
TERMS		 Capripoxvirus. Cell mediated immunity. Vaccines. Viroses.
Proposed Agrovoc immune parameters;
Publication Type Master Thesis
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