studies on the production and use of vero-cells rinderpest vaccine in Egypt
Abstract: Although Plowright rinderpest tissue culture vaccine is absolutely safe and immunogenic but it suffers from several drawbacks since it is prepared on primary cultures (BK cells). Such primary cultures are sophisticated in preparation , expensive, time consuming, restricted in sub culturing, uncontrolled contaminants mediators and containing cell population of various virus susceptibities.
The herein studies have been devoted to avoid the previously mentioned disadvantages b choosing subculturable cell line (VERO cells) and by optimizing the different environmental and cultural conditions in order to get maximum virus titre (potent), easy in preparation, economic, safe, highly immunogenic and cheap vaccine which ended with the following results :
1-37°C as an incubation temperature was proved to be the best to achieve better virus titre than those of 35 and 39°C.
2-The 7.8 pH medium was also the most suitable one to attain further improvement in virus titres.
3-The use of a MOI 3 TCID 100/cell is the much more recommended MOI to get early and significantly increase titres.
4-Virus inoculation on suspended cells instead of a confluent sheet has led to cutting down on the time needed for vaccine preparation with 24-48 hours besides getting of fairly good virus titres.
5-The utilization of rolling method of culturing of Vero cells instead of the use of stationary method has influenced the virus titre positively to give significantly higher virus titres.
6-By making use of environmental and cultural optimum conditions, we managed to prepare three pilot rinderpest vaccine batches on Vero cells having a titre of 106.4 106.6 and TCID50 /ml respectively.
7-The prepared batches proved their freedom of all the contaminant agent (bacterial, fungal and mycoplasma), hence their validity for using as good vaccine.
8-A pool from from the three batches with a titre of 106.5 TCID50/ml was used as an immunizing agent in vaccination of susceptible cattle.
9-The animals that were inoculated either with the field dose as well as those with 100 field dose not exhibit abnormal thermal reaction or any clinical alterations.
10-The immune response of the vaccinated animals, either with the field dose or with the field dose or with the 100 field dose was almost similar. It has been found that the titre in both group of animals (the inoculated ones only) has reached to the undiluted level by the 7th d.p.i and to the level of 15 -25 , 2 w.p.i. and to 150 -200 3 w.p.i.
11- The follow up the immune curve of the vaccinated cattle (both groups) revealed that no further increase in the antibody titres after the 3rd w.p.i.
12-The non-inoculated control animals did not give any rinderpest antibody titre which prove that there was no shedding of viruses the vaccinated cattle to the contact susceptible ones.
It was concluded that the production of rinderpest tissue culture vaccine on VERO cells under the optimum conditions is reliable, safe, potent, highly immunogenic, easy to prepare and store, economic and devoid of the contamination hazards over the preparation on any primary cell cultures (like BK cells).
Publication year 1996
Pages 92-94
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
Country Egypt
Publisher Name: كلية الطب البيطري - جامعة القاهرة
Volume 1 . 1
Author(s) from ARC
External authors (outside ARC)
    محمد سامي صابر استاذ ورئيس قسم الفيروسات - كلية الطب البيطري جامعة القاهرة
    محمد احمد معاذ معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Cells. Morbillivirus. Rinderpest. Vaccines.
Publication Type Master Thesis
↑