Evaluation of Pies Containing Licorice Roots (Glycyrrhiza glabra L.) Extracts

Licorice roots (Glycyrrhiza glabra L.) are an important medicinal plant, and have numerous uses. Two extractors (water and ethanol) were used to extract the phytochemicals from licorice roots. Antioxidant and antimicrobial activities of such extracts were evaluated. Three different concentrations (100, 250 and 500?g/ml) of water and ethanolic extracts were used to investigate inhibition zone and properties against some bacteria strains (Staphylococcus aureus, Salmonella typhimurium, Bacillus subtilis and Escherichia coli) and fungi (Asperigllus flavus, Asperigllus niger and Penicillum chrysogenu). The present results indicated that ethanolic extract exhibited the highest antioxidant activity (AOA) at 600?g/ml. The AOA (IC50) for ethanolic and water extracts was 110 and 212 ?g/ml, respectively. Total phenols of water and ethanolic extracts was 21.01 and 45.24 mgGAE/gDW, respectively. The results of HPLC analysis fractionations showed that both water and ethanolic extracts contained a high level of pyrogallol, coumarin and catechin. The exist major flavonoids were hisperdin and quercetrin in the ethanolic extract. It was revealed that the antimicrobial activity of the ethanolic extract was higher than of the water extract. Three levels of ethanolic licorice extract were used in pie preparing. The effect of addition licorice ethanolic extract (100, 200 and 300 mg/100g wheat flour) in preparing pies were evaluated and the results cleared that, improve in physical properties at 200 and 300 mg/100g compared with control. Taste, volume and crumb color of pies were improved at 200 and 300 mg licorice extract. Addition of ethanolic licorice extract at 200 and 300 mg successfully extended the microbiological shelf life up to 12 days compared with 6 days for control.
Keywords: licorice roots, aqueous extract, ethanolic extract, antioxidant, antimicrobial, bakery products, shelf life.
Publication year 2020
Pages 545-557
Organization Name
serial title Middle East Journal of Agriculture Research
Author(s) from ARC
Publication Type Journal