In-vivo Genotoxicity of the Synthetic Pyrethroid Pesticide -Cypermethrin- in Rat Liver Cells by Comet Assay
Abstract: The comet assay -singe-cell gel electrophoresis, SCGE- is a simple method for measuring deoxyribonucleic acid -DNA- strand breaks in eukaryotic cells. The assay has application in testing different chemical and physical agents for genotoxicity and monitoring environmental contamination with genotoxins. The objective of the present study was to evaluate the genotoxic effects of the synthetic pyrethroid pesticide -cypermethrin-, which is widely used in Egypt in pest-control programs in agriculture and in public health as well. Male rats were sacrificed 1, 7 or 14 days after administration of single oral dose 1/30, 1/10 or 1/5 LD50 of commercial formulation of cypermethrin. Single liver cell suspentions were prepared and Comet assay was performed. With the SCGE assay, a clear induction of DNA damage was observed. It is generally noticide that all pesticide tratments yielded statistically significant -P<0.0001- DNA damage. In conclution, cypermethrin induced a clear significant positive dose-dependent increase in DNA damage in the rat liver cells exposed to cypermethrin. The resuls of the present work suggested that comet assay might be a suitable and sensitive endpoint in genotoxicity evalution of pesticides, but we confirm that various tests should be used for detecting the mutagenic activity of pesticides.
Publication year 2005
Pages 67-82
Availability location CAPLuser(المعمل المركزى للمبيدات)
Availability number
Organization Name
serial title Arab Journal of Biotechnology
Department Mammalian Toxicology
Author(s) from ARC
External authors (outside ARC)
    منى عبد العزيز عبد الحكيم - المعهد القومى لعلوم الليزر، جامعة القاهرة
    يحى عبد الحميد بدر - المعهد القومى لعلوم الليزر، جامعة القاهرة
Agris Categories Pests of plants
AGROVOC
TERMS		 Cypermethrin. Genotoxicity. Pesticides. Rodenticides. Synthetic pyrethrins.
Publication Type Journal
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