Attempts to produce a vaccine against Avian spirochaetosis
Abstract: In this work a lot of efforts had been done for propagation of Brosella anserina microorganism was applied in this present work either by Cuffirating themm in the stablized BSKII culture medium which considered specific medium for Borrelia anserina or by propagating them in the embryonateed chicken eggs via chori-allantoic sac .
Five passages of Borrelia anserina culture were obtained using microaerophilic method of inoculation , while seven passages were recorded by anaerobic route of incubation.
Two types of vaccine adjuvants have been used in the present work which were hydroxid gel (alhydragel) adjuvant and oil (emulsion) adjuvant, good and satisfied results were recorded.
It was found through this present study that the addition of adjuvants to the formalized killed vaccines improve the quality of the vaccine and lead to increase the stability, keeping quality and immunity.
Culture vaccines against Avian spirochaetosis have prepared for the first time in this study.
According to this work, it was found that the culture vaccines have higher immunizing power and longer keeping quality than egg vaccine
Also through this study it was decided that oil emulsion adjuvant provided higher immunity response and more stable thatn hydroxid gel adjuvant.
The efficacy and degree of immunity of the sear of vaccintaed birds wer measured by both ELISA and serum plate agglutination test.
From this study it was found that ELSIA test is very accurate and sensitive.
The prepared vaccines were administred as two divided doses with 3 weeks interval.
Publication year 2005
Availability location مكتبة معهد بحوث الأمصال واللقاحات البيطرية - مكتبة كلية كب بيطري -جامعة القاهرة
Availability number
Organization Name
Country Egypt
Author(s) from ARC
External authors (outside ARC)
    أ.د. زين الدين العابدين رئيس بحوث بمعهد بحوث الأمصال واللقاحات البيطرية
    أ.د. وجيه أرمانيوس جاد السيد استاذ الميكروبيولوجي كلية طب بيطري - جامعة القاهرة
    د. هايدي محمد شوقي استاذ مساعد الميكروبيولوجيا - كلية الطب البيطري - جامعة القاهرة
Agris Categories Animal diseases
Proposed Agrovoc Against avian spirochaetosis;
Publication Type PhD Thesis
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