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Antioxidants Application Enhances Regeneration and Conversion
of Date Palm(Phoenix dactylifera L.) Somatic Embryos
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Abstract: Many embryogenic systems have been designed to generate somatic embryos (SEs) with the
morphology, biochemistry, and vigor uniformity of zygotic embryos (ZEs). During the current investigation,
several antioxidants were added to the maturation media of the developing somatic embryos
of date palm. Explant material was a friable embryogenic callus that was placed in maturation media
containing ABA at 0.5 mg L??1, 5 g L??1 polyethylene glycol, and 10 g L??1 phytagel. Furthermore,
-tocopherol or reduced glutathione (GSH) were used separately at (25 and 50 mg L??1). These
treatments were compared to a widely used date palm combination of reduced ascorbic acid (ASC)
and citric acid at 150 and 100 mg L??1, respectively, and to the medium free from any antioxidants. The
relative growth percentage of embryogenic callus (EC), globularization degree, differentiation%, and
SEs number were significantly increased with GSH (50 mg L??1). Additionally, the latter treatment
significantly enhanced the conversion% of SEs and the number of secondary somatic embryos (SSEs).
ASC and citric acid treatment increased leaf length, while -tochopherol (50 mg L??1) elevated the
number of leaves plantlet??1. GSH at 50 mg L??1 catalyzed the activities of polyphenol oxidase (PPO)
and peroxidase (POD) in EC and enhanced the accumulation of proteins in SEs.
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| Publication year |
2022
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| Organization Name |
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| Author(s) from ARC |
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| Publication Type |
Journal
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