Further studies on haemorrhagic viral disease in rabbits in Egypt
Abstract: Sixty one suspected RVHD outbreaks were monitored and diagnosed preliminary in Egypt. The preliminary diagnosis was based on flock history, clinical examination, PM examination, histopathological examination, HAT and virus isolation. RVHD diagnosis was confirmed by carrying out of , SDS-PAGE, Western blotting, RT/PCR assay and IEM. SDS-Page and Western blotting demonstrated the presence of a major antigen of molecular weight of 50 to 55 KDa approximately. RHDV isolates were tested by RT-PCR using specific primers of three genomic regions encoding the capsid protein (VP60) (regions A and B) and none structural protein (region C), giving positive results. Amplification of VP60 region B from 3 RHDV Egyptian strains, EgyptGiza97, Egypt KS 2000 and Egypt Kalubia 2000, yielded PCR products of 500 bp. After sequencing of these products, the sequences were submitted to GenBank with accession number EF488823, EF 488824 and EF 437942 respectively. Amplification of VP60 region A of EgyptKS 2000 yielded PCR product of 500 bp too. After sequencing of this product, the sequence was submitted to Genbank with accession number EF22287. The obtained nucleotide and amino acid sequence data were compared with that of reference strains of 17 published on Genbank RHDV sequences, one RCV strain and one EBHSV. RHDV Egyptian strains EgyptGiza 97 and EgyptKalubia2000 were genetically and antigenically related and grouped within one genogroup, while RHDV Egyptian strain EgyptKS2000 was genetically different and segregated into different genogroup but antigenically related on the level on the level of VP60 region B.RHDV Egyptian strain EgyptKS2000 was genetically grouped to group 1, so it is related genetically to the other 2 Egyptian strains, while this strain is antigenically different. The pathogenicity of most recently isolated RHDV code no. R58 (Giza2006) of HA titer 214 HAU) was carried out using susceptible rabbits. An inactivated vaccine against RVHD was prepared from the most recently local isolate (Giza 2006) which identified through HAT, IEM and LD50. Comparative study between the local recent inactivated RHDV vaccine and the imported one was carried out. The local vaccine proved to be more immunogenic and protective than the imported one.
Publication year 2007
Pages 1-3
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
Country Egypt
Publisher Name: كلية الطب البيطري - القاهرة
Volume 1 . 1
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Haemorrhagic septicaemia. Haemorrhagic septicaemia virus. Rabbits. Viroses.
Publication Type PhD Thesis
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