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Using biotechnology in maintenance of genetic resources of local breeds of rabbits
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Abstract: A total of 30 New Zealand White (NZW) and 30 Baladi Black (BB) rabbit does was used as donors of oocytes as well as 6 fertile bucks, (3 NZW and 3 BB) were used for semen collection of achieving in vitro fertilization in this study.
The present study included three experiments.
1.EXPERIMENT I:
The aim of this experiment was to evaluate the effect different sources and levels of antioxidants on in vitro maturation NZW and BB rabbit oocytes. In this experiment, a total of 20 NZW and 20 BB rabbit does was slaughtered as oocyte donors. After slaughter, ovarian characteristics of each doe including weight and number of follicles, oocytes were collected using slicing technique. After slicing, oocytes recovered were washed, examined under stereomicroscopy and classified according to their compaction, number of cumulus cell layers and homogeneity of ooplasm. Five types of maturation medium (TCM-199) were used for in vitro maturation, including TCM-199 supplemented with 2 or 3 mM of ascorbic acid and 0.05 or 1 mM of reduced glutathione
(C10H17N3O6S) (GSH) as compared to control medium. After 20 has a maturation period, oocytes were washed, loaded on clean slide, stained and examined for maturation under phase-contrast microscopy.
2. EXPERIMENT II:
This experiment aimed to determine the effect of different sources and levels of capacitation substance on in vitro capacitation of NZW and BB rabbit spermatozoa. Semen ejaculates were collected and the jelly mass of each ejaculate was discarded and net volume of semen was measured. Each ejaculate was evaluated for progressive motility, live sperm, sperm abnormality and sperm cell concentration.
A total of 7 types of capacitation media was prepared and supplemented with 6 levels of capacitation substances including 25, 30 and 35 µg/ml of heparin and 25, 50 and 75 µm/ml of calcium ionophor in addition to the control medium without supplementation was used for sperm capacitation.
Semen of three bucks from each breed on each collection day was pooled and assigned into 7 portions for each level of capacitation substance. Acrosome reaction test was conducted for each source and level of capacitation substance and different forms of spermatozoa detected during the acrosome reaction were recorded.
3. EXPERIMENT III:
Based on the best results of source and level of antioxidant during in vitro maturation and the type and level of in vitro capacitation substance, in vitro fertilization was conducted in this experiment. Fertilizing rate after 24 hand developmental competence of embryos at different embryonic stages after 72 h was investigated.
In conclusion. the present study indicated that:
1-There were significant differences between NZW and BB in ovarian
weight and number of follicles or oocytes per ovary or doe, being significantly higher in BB than in NZW breed However, oocyte recovery rate was not affected by rabbit breed, being 88.34 and 90.16% in NZW
and BB breeds.
2- Supplementation of TCM-199 with AA had a beneficial effect on in
vitro maturation of rabbit oocytes; and that can be achieved at levels of 2 mM for NZW and 3 mM supplemental AA for BB oocytes.
3- Semen collected from BB bucks was of good quality than that obtained from NZW bucks under the Egyptian condition.
4- Heparin supplementation at a level of 35 µg/ml had beneficial effect
rather than other heparin levels or all calcium ionophor levels on sperm capacitation used for in vitro fertilization for NZW and BB breeds.
These findings may indicate the beneficial effects of antioxidant supplementation of in vitro maturation medium with different sources and
levels on in vitro fertilization rate of BB and also NZW breed rather than effects of antioxidants on in vitro development of embryos.
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| Publication year |
2012
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| Pages |
PP. 152
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| Availability location |
مكتبة معهد بحوث الانتاج الحيوانى- شارع نادى الصيد - الدقى- الجيزة
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| Availability number |
1194
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| Organization Name |
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| Country |
Egypt
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Antioxidants.
In vitro fertilization.
Ova.
Rabbits.
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| Proposed Agrovoc |
in vitro maturation;local breeds;
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| Publication Type |
PhD Thesis
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