Micropropagation and Biomass Production of Rubus fruticosus L. (Blackberry) plant
Abstract: Blackberry is considered one of the most industrial plants, while a large amount of its fruits is
sold as fresh another has multiplied biomass production, such as flavonoids, vitamin C, polyphenolic
and antioxidants which inter in medicinal and food processes. Therefore, this investigation has been
carried out to study the micropropagation of Rubus fruticosus shootlets, induction and growth of
callus and enhancement of previous bioproduct content in both of shootlets and calli. The results
revealed that free MS medium gave the highest significant values in survival (91.67%), shootlet
(1.83), leaves numbers/explant (7.33) and shootlet length (31.67mm) in the establishment stage
compared with WPM or B5 media. Multiplication medium provide with BA at 0.6 mg/l recorded the
highest survival (100%) and greatest number of shootlet /explant (3.4) after the first subculture
compared to other treatments. Adding activated charcoal at 2g/l to quarter strength of MS or the
culture on half MS strength gave the maximum rooting (100%). In acclimatization stage, transplanting
the plantlets in green house on mixture soil of peatmoss only or of (1peatmoss:1sand /v/v) was the
most suitable for raising the survival of plantlets to (100%). For callus induction, the increase in
concentration of 2, 4-D from 0.5 to 1.0 mg/l on callus medium plus 0.5 mg/l NAA raised the rate of
callus formation by (100%). The combination between 0.5mg/l NAA and 1.0mg/l 2, 4-D gave the
highest value of callus fresh weight across three subcultures. The highest content of bioactive
compounds obtained from shoots represented as chlorophyll a, chlorophyll b, total carotenoids, total
phenols and total antioxidant (112.26, 46.57, 127.72, 239.45 and 948.61 mg/100g), respectively were
recorded as result of using 0.4 mg/l BA. While, using 0.2mg/l BA in the culture medium resulted the
highest amount of total flavonoids and vitamin C (28.50 and 222.30 mg/100g FW), respectively.
URL
Publication year 2018
Organization Name
serial title Middle East Journal of Applied Sciences
Author(s) from ARC
Publication Type Journal
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