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Resistance monitoring and enzyme activity in three field collected populations of Cowpea aphid (Aphis craccivora) from Egypt
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Abstract: Cowpea aphid (Aphis craccivora) is one of the most important sucking insect pest attacking faba bean, Cowpea and pea. In this study we determined the efficacy of seven insecticides against three field strains collected from three Governorates ;Dakahlia (D), Qalyobia (Q) and Beni sweif (B). Diagnostic concentrations (LC90 values of S-strain) for each insecticide were established by dose response assay using leaf dipping technique. Resistance monitoring studies showed that field strain from Dakahlia Governorate was highly detected to the tested organophosphates, carbamates and neonicotinoids. However, the field strain from Bani-Sweif exhibited lower level of resistance to the tested insecticides
Esterase activity in the three field strains (D,Q and B) was generally high as activity ratio ranged between7.8- 4.3. Whereas, the activity of both glutathione –s- transferase and mixed function oxidases were moderate in Q and D field strains. However, in the Beni-Sweif strain , the enzyme activities were varied and slightly different from S strain. Resistance monitoring and enzyme activity investigated in this study proposed that the resistance in Cowpea aphid to the tested insecticides may be due to the higher activity of the metabolic enzymes namely carboxylesterases.
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2015
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Journal
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