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Effect of probiotic supplementation on DNA Fragmentation, some Hematological, Biochemical and Antioxidant parameters in sheep
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Abstract:
Summary
This study was conducted to evaluate the effect of probiotic supplementation to ewes previously exposed to under nutrition on DNA fragmentation, enzymatic and, non enzymatic antioxidant as well as some hormones and biochemical parameters. Fifteen sexually mature local ewes of 2-3 years old during the breeding season in a private farm at Giza Governorate. Ewes were used in the current study free from any infectious diseases and external parasites. All ewes were synchronized by impregnated sponge divided into three groups.The first group under nutrition group, second group re-fed on balanced ration and third group re-fed on balanced ration supplemented with Ru-max 3gm/head /day for two months, one month before synchronization and one month after. Blood samples were collected at zero day and 9 day of estrous cycle to investigate DNA fragmentation percentage, the oxidative stress and some hemato- biochemical changes
Hematological examination showed significant increase in RBCs count, Hb, PCV. and TLC at (p<0.05) in ewes supplemented with proboitics compared to other groups . There is a significant decrease at (P<0.05) in DNA fragmentation percentage and bands intensities accompanied by significant decrease of lipid peroxidase activity and nitric oxide with significant increase in the activities of the glutathione–S-transferase ,SOD and catalase (enzymatic antioxidant ) and vitamins (A,C,E) and trace elements copper , zinc, selenium and iron (non enzymatic antioxidant) in probiotics supplemented ewes compared to other group Probiotic supplementation significantly increased at (P<0.05) total protein, albumin , globulin and glucose concentrations accompanied by non significant difference between groups in serum urea creatinine, ALT and AST. There was a significant increase p (<0.05) of blood insulin and reproductive hormones in probiotics supplemented ewes than other groups, also, there was significant improve in level of progesterone and estradiol hormones of probiotics supplementation at p (<0.05) .
In conclusion, addition of Ru-max as a biological feed additives to the ewes in case of stress due to under nutrition had positive and beneficial effects to enhance digestion and nutritive values, rumen fermentation, while it suppress oxidative stress which consequently decrease fragmentation of DNA and improved ovarian activity by its positive effects on metabolic hormones and blood constituents without any adverse effect on either liver or renal functions
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| Publication year |
2013
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| Pages |
مقبول النشر
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| Availability location |
المجلة
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| Agris Categories |
Animal physiology and biochemistry
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| Publication Type |
Researsh & Applied Activities
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