Development of an in vitro regeneration system from immature inflorescences and CRISPR/Cas9?mediated gene editing in sudangrass

Abstract: Background Sudangrass (Sorghum sudanense) is a major biomass producer for livestock feed and biofuel in many countries. It has a wide range of adaptations for growing on marginal lands under biotic and abiotic stresses. The immature inflorescence is an explant with high embryogenic competence and is frequently used to regenerate different sorghum cultivars. Caffeic acid O-methyl transferase (COMT) is a key enzyme in the lignin biosynthesis pathway, which limits ruminant digestion of forage cell walls and is a crucial barrier in the conversion of plant biomass to bioethanol. Genome editing by CRISPR/Cas9-mediated mutagenesis without a transgenic footprint will accelerate the
improvement and facilitate regulatory approval and commercialization of biotech crops.
Publication year 2023
Organization Name
serial title Journal of Genetic Engineering and Biotechnology
Author(s) from ARC
External authors (outside ARC)
    M. H. Abd El-Aziz Genetics Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt
Publication Type Journal