Production and evaluation of Anti rift valley fever (RVF) IgG conjugated with fluorescein
Abstract: Three susceptible sheep were inoculated with RVF antigen mixed with incomplete and complete Freund's adjuvant and without adjuvant in three successive doses. The hyperimmune sera collected from the sheep were tested against standard reference RVF antigen for the presence of specific RVF antibodies using serum neutralization test (SNT), agar gel precipitation test(AGPT)and indirect fluorescent assay (IFA). Separation of RVF immunoglobulins were done uaing affinity chromatography (activated sepharose 4B) followed by conjugation of RVF IgG with fluorescen isothiocyanate at pH 9.6 . The conjugated RVF antibodies were titrated against reference RVF antigen and the titre reached (1:128). The working dilution was used for evaluation of different batches of attenuated and inactivated RVF vaccines in comparison with IFA test using anti-sheep Fitic, the result of both assays proved to be simiar.
Publication year 2001
Availability location معهد بحوث الامصال واللقاحات البيطرية - ش السكة البيضاء - العباسية - القاهرة
Availability number
Organization Name
City القاهرة
serial title Egypt. J.Agric, Res, 79(4), 2001.
Author(s) from ARC
External authors (outside ARC)
    فريد فؤاد زكي
Agris Categories Animal diseases
AGROVOC
TERMS		 Antiaris. Evaluation. Fluorescence. Rift valley fever virus.
Publication Type Journal
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