Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitro
Abstract: The effect of ovarian status and steroidogenic potential of granulosa cells and/ or follicular fluid on the maturation of camel oocytes in vitro during breeding and non-breeding seasons were studied. The camel ovaries were collected immediately after slaughter. The oocytes were aspirated from follicles with 8 - 20 mm diameter for culture in maturation medium (MM) under different co-culture conditions; MM+No GCs (Maturation Medium + No Granulosa Cells), MM+FGCs (Maturation Medium + Fresh Granulosa Cells), MM+PGCs (Maturation medium + Pre-incubated Granulosa Cells), MM+ 10%FF (Maturation Medium + 10% Follicular Fluid) and MM+FGCs+ 10%FF. The obtained results revealed that, the distribution of small follicles on ovaries was significantly (P<0.0l) higher than the medium and pre-ovulatory follicles. The different follicular populations with or without corpora lutea were significantly (P<0.0l) higher during breeding than non-breeding season. During each season, the numbers of follicles were higher on ovaries with than without corpora lutea. There were no significant differences (P<0.01) in the numbers of medium and large atretic follicles when two seasons were compared, whereas the medium atretic follicles showed higher significant rate (P<0.0l) than large follicles during both seasons. The mean value of grad 1+2 oocytes was significantly (P<0.0l) higher than grad 3+4 oocytes only during breeding season, although the two groups were higher during breeding season than non-breeding one.
The highest rates of metaphase-II oocytes were observed after co-culrure with FGCs during breeding and non-breeding seasons, whereas the lowest rates were observed after co-culture with PGCs or FF. In all groups, the rates of metaphase-II oocytes were higher during breeding season than non-breeding one. The rate of nuclear maturation increased progressively with the duration of co-culture reaching the highest at 36 hrs during breeding season. During non-breeding season, the rate of nuclear maturation increased from 12 to 24 hrs and. remained constant up to 36 hrs.
The potential of FGCs or PGCs to secrete testosterone and estradiol-17β in culture media was significantly (P<0.0l) increrased with the duration of coculture reaching the highest with FGCs+FF at 36 hrs, with exception the level of estradiol-17β decreased after co-culture with PGCs. The potential of FGCs or PGCs to secrete progesterone increased progressively with the duration of cocultring reaching the highest with PGCs at 36 hrs. The level of three hormones maintained significantly (P<0.0l) higher during breeding than non-breeding season. In conclusion, conditioning of the culture media with GCs and FF had the potential to mature camel oocytes during non-breeding as well as breeding seasons. This will increase and improve the in-vitro fertilization and embryo transfer programme in one-humped camel.
Publication year 2003
Pages 89-109
Availability location معهد بحوث الانتاج الحيوانى - شارع نادى الصيد- الدقى - الجيزة
Availability number
Organization Name
City المنصورة
serial title Mansoura Veternairy Mededical Journal
Author(s) from ARC
External authors (outside ARC)
    حسين احمد عامر جامعة الزقازيق
    سامى معوض زعبل جامعة المنصورة
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Breeding seasons. Camels. Fluids. Granulosa cells. In vitro. Maturation. Ova. Ovarian cysts.
Proposed Agrovoc steroidogenic potential;
Publication Type Journal
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