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EFFECT OF SILVER NANOPARTICLES ON MICROPROPAGATION OF
DATE PALM (PHOENIX DACTYLIFERA L, CV. SEWI AND MEDJOOL)
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Abstract: The current research was carried out to assess the potential effects of silver nanoparticles on contamination and in vitro
development of date palm, immature inflorescences explants of Sewi and Medjool date palm were cultured on MS medium
supplemented with 1mg L-1 2,4-D, 1mg L-1 2iP and 1g L-1 activated charcoal. Silver nanoparticles treatments (0, 125, 250 and
500?g L-1) were applied during establishment, callus differentiation and multiplication stage. Contamination%, direct somatic
embryos%, callus%, callus growth degree, average number of embryos per cluster and number of shoots per cluster were
recorded. The obtained results showed that silver nanoparticles has a good potential for removing contaminants in date palm
tissue culture, 500?g L-1 of silver nanoparticles considered as the most successful disinfection treatment, which decreased
contamination to 2.77% compared with 19.44% in control treatment during establishment stage and recorded zero during
multiplication & germination stage. Regarding number of embryos and shoot number, the lower concentrations of AgNPs
gave better values compared with higher concentration while, the highest callus formation, callus growth and globularizaton
were recorded at the higher concentrations of silver nanoparticles.
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| Publication year |
2020
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| Organization Name |
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| Author(s) from ARC |
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| Publication Type |
Journal
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