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High-frequency direct somatic
embryogenesis and plantlet regeneration
from date palm immature inflorescences
using picloram
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Abstract: Background: Date palm (Phoenix dactylifera L.) is a traditional crop in arid and semi-arid areas. Its vegetative
propagation can be achieved by offshoots, but possible number of offshoots in mother palm trees is limited.
Micropropagation is a highly recommended strategy for obtaining date palm elite cultivars using shoot tip and
immature inflorescences. In this study, micropropagation procedure using inflorescence explants of Medjool cv. is
described. For culture initiation, explants from different spathe lengths were cultivated on Murashige and Skoog
medium (MS) supplemented with picloram at 1.0 and 2.0 mg/l combined with 2iP at 0.5 mg/l alone and with both
2iP and BA at 0.25 mg/l for 24 weeks. The obtained direct globular embryos were transferred to maturation media
with 0.1 mg/l picloram alone or combined with both 2iP and ABA separately and together for further development.
Additionally, multiplication and rooting media were optimized by different cytokinins and auxins for high frequency
of plantlet production. Acclimatization of in vitro plantlets was also investigated.
Results: The highest percentage of globular embryo formation was noticed with explants isolated from spathe
lengths ranging from 10 to 15 cm. Addition of BA to initiation media with picloram encouraged a significant effect
on embryonic culture formation percentage. Incorporation of ABA and 2iP to maturation medium was an effective
factor for individual or multiple embryo emergence. Acclimatization of in vitro plantlets having 3–4 roots was
successfully accomplished. Irrigation with the full strength solution (MS) encouraged the highest growth vigor
degree, leaf number/plant, leaf width, root number, and root thickness degree of ex vitro plants.
Conclusion: This research provides an advanced regeneration system for large-scale production of date palm from
immature inflorescences of Medjool cv. It opens up the prospects of using picloram with different growth
regulators for rapid micropropagation of date palm
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| Publication year |
2019
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| Organization Name |
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| Author(s) from ARC |
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| Publication Type |
Journal
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