Thidiazuron-induced direct organogenesis from immature inflorescence of three date palm cultivars
Abstract: Background: Inflorescence explants of date palm proved to be a promising tool for micropropagation of elite
cultivars or rare males and females as organogenesis and somatic embryogenesis could be achieved. These plant
materials are abundantly available every year and can be used as cheap and potent explants. Nevertheless, many
difficulties could be faced in this protocol according to selection of the spathe size and age, media components,
growth regulators, etc. The aim of this study was to determine the influence of various cytokinins on direct organs
induction of three date palm cultivars (Selmi, Barhee, and Medjool) from immature inflorescence. An additional
objective of this study was to investigate the effect of cytokinins and auxins on growth and development of
Medjool cultivar.
Results: Various combinations of cytokinins were investigated on three date palm inflorescences as N6-(2-
isopentenyl) adenine (2iP), kinetin, benzyleadenine (BA), and thidiazuron (N-phenyl-N?-1,2,3-thidiazol-5-yl urea) (TDZ).
TDZ alone or in combination with BA proved to be superior for direct organogenesis in all three cultivars so that
another combination of TDZ with BA was conducted. Results showed that moderate concentration of BA, with TDZ,
gave superior response. Medjool cultivar response surpassed other two cultivars that made the possibility to
conduct some growth regulators treatments on its multiplication and regeneration. TDZ at 0.5 + BA at 1.0 mg/l
without activated charcoal seemed to enhance multiplication rate. Medium containing 0.5 mg/l of both
naphthaleneacetic acid and indole butyric acid in addition to 1.0 mg/l indole acetic acid appeared to be more
suitable for rooting stage of Medjool shootlets.
Conclusion: In this study, we created an innovation sequence of growth regulators included in nutrient media for
date palm direct organogenesis from inflorescence. Organogenesis has been accelerated from immature
inflorescence explants and developed to healthy plantlets which acclimatized in greenhouse.
Publication year 2021
Organization Name
Author(s) from ARC
Publication Type Journal
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