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Biochemical characterization of
micropropagated Ceratonia siliqua L. under
effect of growth regulators and light
quality
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Abstract: Background: Carob (Ceratonia siliqua L.) is one of the important crops in terms of nutritional and medicinal value
in the countries of Western Asia and North Africa. Despite the fact that some countries have used Carob in
traditional medicines, the modern food industry began to discover the great potential of this plant which
differentiates with antioxidant capacity as well as other important medicinal activities. Some of very few studies that
have been reported on this important plant were published.
Methodology: In the present study, micropropagation behavior and biochemical constituents of Ceratonia siliqua L.
cultured on MS medium supplemented with different types and concentrations of cytokinins (0.0, 0.5, 1.0, or 1.5
mg/l BA, Kin, or TDZ and 0.2 mg/l NAA) under various light qualities ((white, green, blue, red, and yellow) were
examined.
Results: MS culture medium supplemented with BA produced the highest shootlets number/explants. For light
quality, the data showed that green light increased shootlet number/explants, fresh and dry weights. However,
yellow light increased shootlet length and leaves number and also caused significant increase in total phenols,
flavonoids, and antioxidant capacity (DPPH) as compared to other lights. HPLC analysis showed that yellow light
caused the highest accumulation of total phenolic and catechin that were the highest accumulated compounds
(509 ?g/ml) followed by that were accumulated under green light (412.68 ?g/ml). Shootlets grown under white
light (control) gave the highest accumulation of p-coumaric acid, rosmarinic acid, and cinnamic acid. Shootlets
grown under blue light gave the highest accumulation of gentisic acid and syringic acid. Red light caused the
highest accumulation of vanillic acid. However, white light accumulated the highest amount of flavonoid
compounds comparing with other light qualities.
Conclusion: This study had reached to optimize a suitable micropropagation protocol of Ceratonia siliqua L. and
characterization of biochemical constituents that can be beneficial for increasing its medical value which will help
in food industries for commercial purposes
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2020
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Journal
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