Effect of pregnancy status and harvesting technique on recovery rate and categories of dromedary camel oocytes
Abstract: The present study was carried out to investigate the effect of harvesting technique and pregnancy status on recovery rate and categories of camel oocytes. Ovaries were collected from local slaughterhouse in pairs per animals (pregnant or non-pregnant) with determined the site (right and left) two harvesting techniques were used to collect oocytes (aspiration and slicing techniques). The oocytes were evaluated microscopically and classified into six categories. Results revealed that ovaries were heavier (P>0.001) in pregnant than non-pregnant camels (7.1 vs. 4.1 g). Overall mean of number of follicles/ovary was 12.71 /ovary. and did not differ significantly in pregnant than non-pregnant (13.56 and 11.82/ovary respectively) and on right and left ovaries (13.0 and 12.4/ovary) the greatest number of follicles/ovary was obtained from right ovaries of pregnant camels ( 14.10/ovary).While the lowest values were obtained from left ovaries of non-pregnant camels. Overall number of oocytes/ovary was 3.99/ovary, being higher (P>0.01) by 55% with slicing than aspiration and in non-pregnant than pregnant (3.96 vs. 4.0] /ovary, P<0.05). Overall mean of recovery rate was 31.4%. being insignificantly higher with slicing than aspiration (28.5 vs. 37.42%) and in non-pregnant than pregnant camels (29.20 vs. 33.91%) the highest number of oocytes/ovary (5.81/ovary) and recovery rate (37.71%) were recorded when oocytes recovered from ovaries of non-pregnant camels using slicing technique. Frequency distribution of the normal oocytes with 1-4 and > 4 cumulus cell layers averaged 50.48%, being higher with aspiration (52.3%) than slicing (47.53%). and in pregnant (51.7%) than non-pregnant (49.2%) camels. The highest distribution of normal and good quality oocytes (54.8%) was obtained when aspiration technique was achieved from ovaries of pregnant camels.
Key words: Camel. Follicles. Oocytes. Aspiration. Slicing. Recovery Rate.
Publication year 2007
Pages 255-268
Availability location معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
Availability number
Organization Name
serial title Egyptian Journal Basic .Applied Physiology
ISSN 1687-1146
Author(s) from ARC
External authors (outside ARC)
    مصطفى عبد الحليم الحرايرى جامعة المنصورة
    عبد الخالق السيد عبد الخالق جامعة المنصورة
    وائل احمد خليل جامعة المنصورة
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Cutting. Dromedaries. Ova. Pregnancy. Technology.
Proposed Agrovoc Follicles;Aspiration;Recovery Rate;
Publication Type Journal
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