Effect of ovary preservation period on recovery rate and categories of dromedary camel oocytes
Abstract: The aim of this study was to evaluate the effect of different periods of ovary preservation at 25-30ºC for 5, 6, 7, 9, 12 and 24 hours on recovery rate and oocyte categories of dromedary camel oocytes. Camel ovaries were collected from El- Bassatein slaughterhouse, Cairo. The collected ovaries were placed immediately after slaughtering into thermos in saline solution (0.9% NaCI) supplemented with antibiotics (100 IU penicillin and 100μg streptomycin/ml) at 25-30ºC and transported to the laboratory within 4-5h. Ovaries were washed three times with warmed (30°C) phosphate buffer solution (PBS) and one time with ethanol (70%). All visible follicles on the ovarian surface (2-8 mm in diameter) were counted. Oocytes were aspirated using a 20-gauge hypodermic needle. Oocyte yield was recorded and the number of oocytes/ovary was calculated. Oocytes were classified into five categories (compact-partial denuded- denuded- shrunken and cleaved oocytes). Results show that average number of follicles on each ovary was not significantly affected by preservation period, although tended to reduced only after 5 hours of ovary preservation. However, this number was insignificantly reduced by increasing period of ovary preservation more than 5 up to 24 hours. Average number of oocytes on each ovary was significantly (P<0.05) reduced only between 5 and 6 h of ovary preservation. Average number of oocytes showed higher reduction rate between 5 and 6 hours from 12.4 to 9.3/ovary as well as between 9 and 12 hours. Oocyte recovery rate showed insignificant decrease from 88.1 % at 5 h to 78.6% at 9 h of preservation. However, it showed significant (P<0.05) reduction to 62.0% between 9 and 12 h, then insignificantly decreased to 58.6 at 24 h of preservation of the ovaries. Frequency distribution and recovery rate of each category was the highest for compact oocytes and the lowest for cleaved oocytes at all periods of preservation. Increasing preservation period significantly (P<0.05) decreased frequency distribution of compact and cleaved oocytes, while increased frequency distribution of partial denuded, denude and shrunken oocytes. In conclusion from the present results the preservation of dromedary camel ovaries at 25-30 ºC for 5-6h was effective for maintaining the oocytes quality and recovery rate compared with the other preservation periods.
Keywords: Camel, ovary, preservation, oocyte, recovery rate, category.
Publication year 2008
Pages 6443-6451
Availability location معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
City المنصورة
serial title Journal Agriculral Science Mansoura University
ISSN 1110-0346
Author(s) from ARC
External authors (outside ARC)
    عبد الخالق السيد عبد الخالق جامعة المنصورة
    مصطفى عبد الحليم الحرايرى جامعة المنصورة
    وائل احمد خليل جامعة المنصورة
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Camels. Dromedaries. Ova. Ovaries. Preservation.
Proposed Agrovoc categories;recovery rate;
Publication Type Journal
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