Recovery rate of follicular and oviductal oocytes of doe rabbits super-induced to ovulation by hormonal treatments
Abstract: This study was carried out. at the in vitro fertilization (NF) Laboratory, International Livestock Management Training Center (ILMTC), Sakha, Kafr El-Sheikh Governorate, belonging to the Animal Production Research Institute, Ministry of Agriculture. Agricultural Research Center on 35 New Zealand white (NZW) rabbit does (5-6 mo of age and 3.0-3.5 kg LBW) to evaluate the effect of different hormonal treatments, using PMSG and hCG or GnRH on recovery rate of follicular and oviductal oocytes. Does in the 1st group (G1) were i.m. injected with GnRH and slaughtered after 12 h from GnRH injection (control group). However. does in G2. G3 and G4 were i.m. injected with 75 IV of PMSG analogue (Foligon). Rabbit does were injected 48 h later by 150 IU of hCG (G2), 48 h later by 0.02 ml GnRH analogue (Receptal, G3) and 72 h later by GnRH injection (G4). Does in all groups were slaughtered 12 h from the last injection for oocyte collection. Mature oocytes were collected from oviduct. Number of corpora lutea (CLs) and follicles more than 1 mm in diameter were recorded on each of left and right ovary of each doe. Oocytes were collected from ovaries using slicing technique. Follicular and oviductal oocytes were classified into defferent categories. Results revealed significant (P<0.05) differences in number of oocytes recovered from ovaries or oviducts per right and left side or per doe and in number of corpora lutea on the ovaries between experimental groups, being the highest in G3. The highest recovery rate from ovaries was obtained in G3 (91.2%) as compared to 85.7% in G1, 83.3% in G2 and 81%% in G4. The highest recovery rate from oviduct was obtained in G4 (92%) as compared to 82.8% in G3 and 82.4% in G1. H6wever, no oocytes were recovered from oviduct of G2. Total recovery rate of oocytes from ovaries and oviducts was the highest in G3 (87%), followed by GG4 (86.5%) and G1 (84.4%), and the lowest in G2 (82.3%). Percentage of compact oocytes recovered from the ovaries was the highest in G3, ranked the second in G2 and the third in G4, being 89.6, 86.0 and 81.3%, respectively. H6wever, G1 showed the lowest percentage (55.6). In all groups, frequency distribution of oocytes at M II was the highest as compared to those at MI or degenerated oocytes. percentage of oocytes at M II was lower in G3 and G4 than in G1. being 83. 78 and 86%. respectively
According to the obtained results treatment of rabbit does to stimulate over ovulation by PMSG analogue (75IU) followed 48 h later by GnRH analogue (0.02 ml) and slaughtered 12 h from the last injection yielded the highest number of compact 00-cytess(immature) recovered from the ovarian follicles and acceptable number of mature oocytes from oviduct.
Keywords Rabbits . oocytes. Ovarian characteristics recovery rate. oocyte categories.
Publication year 2008
Pages 133-148
Availability location معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة.
Availability number
Organization Name
City المنصورة
serial title Mansoura, Veterinary Medical Journal
ISSN 1110-7219
Author(s) from ARC
External authors (outside ARC)
    عبد الخالق السيد عبد الخالق جامعة المنصورة
    ترك ابراهيم درة جامعة المنصورة
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Hormones. Ova. Ovarian cysts. Ovulation. Rabbits.
Proposed Agrovoc oviductal oocytes;Ovarian characteristics reco;oocyte categories;
Publication Type Journal
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