Pellet-freezing of Damascus goat semen in a chemically defined extender
Abstract: During the breeding season of goats (12 bucks and 64 does) in Egypt, five experiments were conducted using a chemically defined cryoextender (CDE) to investigate: (I) the influence of rates of semen dilution (1 :2, 1:4 and 1: 19) and methods of thawing of frozen semen pellets (dry thawing versus wet thawing) on spem progressive motility (SPM), spem acrosome abnormalities (SAA) and rate of lipid peroxidation in semen as measured by malonaldehyde (MAL) production, and (2) the effect of insemination of does in natural (n = 38) and cloprostenol-synchronized (n = 26) estrus with frozen semen on their kidding rates and prolificacy. Semen (two successive ejaculates/buck) was collected twice a week via an AV and only ejaculates of>2500 x 106 spenn/ml and 70% SPM were diluted in one step at 30 °C with the CDE, cooled to 5 °C over a 4 h-period, frozen in the form of 0.30 ml pellets and stored in liquid nitrogen for 72 h. The results revealed that post-thaw SPM of semen diluted at a rate of 1:4 was significantly (p < 0.01) higher than that of semen diluted at the other rates. Dilution of semen at a rate of 1: 19 ( ≤151 x 106 sperm/ml) not only minimized (p < 0.0 I) pre-freeze and post-thaw SPM, but also augmented (p < 0.01) pre-freeze and post-thaw rates of lipid peroxidation as evidenced by the high level of MAL production and the ability of antioxidants (1 mg/ml EDTA, 200 U/ml bovine liver catalase, 0.61 mg/ml reduced glutathione and 0.11 mg/ml sodium pyruvate) to restore (p< 0.01) pre-freeze and post-thaw SPM. Frozen semen pellets exposed to dry thawing had a greater percentage of SPM (p< 0.01) as well as lower values of SAA and MAL (p<0.01) than those exposed to wet thawing. Although the kidding rates did not vary significantly among does in natural (55.26%) and synchronized (53.85%) estrus, a higher (P< 0.05) prolificacy was obtained after their insemination in natural (1.81 ± 0.16) rather than in synchronized (1.22± 0.11) estrus.
© 2005 published by Elsevier B.V.
Keywords: goat; frozen semen; Chemically defined extender
Publication year 2006
Pages 305-315
Availability location معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
serial title Animal Reproduction Science
Author(s) from ARC
External authors (outside ARC)
    طارق خليفة جامعة المنصورة
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Goats. Semen. Semen preservation.
Proposed Agrovoc Pellet-freezing;chemically defined extender;frozen semen;
Publication Type Journal
↑