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Comparative studies between traditional and molecular diagnosis of mastitis in goat
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Abstract: The primary causes of mastitis in cattle, goats and sheep are well-recognized groups of microorganisms as Staphylococcus spp.; Streptococcus spp.; Pasteurella spp.; Escherichia coli; Enterobacter spp. and Klebsiella spp. The objective of this study was to develop a Multiplex Polymerase Chain Reaction (MPCR) method for simultaneous detection of Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus uberis directly from milk samples either positive or negative to California test, using specific primers for each microorganism. Two hundred and fifty milk samples represent 125 goats were tested by California test and submitted for isolation of different microorganism using traditional methods. S.aureus, S.agalacteae, S.ubris, E.coli and C. pyogenes were isolated from examined milk samples in different percentages. The results were discussed. Thirty two milk samples were chosen to represent milk samples analyzed by traditional methods were submitted to be examined by MPCR for diagnosis S. aureus, S. agalactia, and S. uberis. Results of conventional microbiological methods were used to determine the sensitivity and specificity of the multiplex PCR. Results of this study indicate that the multiplex PCR procedure has the potential to be a valuable diagnostic technique for simultaneous identification of S. aureus, S. agalactiae, and S. uberis directly from milk samples specially to diagnose subclinical methods.
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| Publication year |
2009
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| Pages |
564-581
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
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| Availability number |
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| Organization Name |
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| City |
بنها
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| serial title |
Benha Veterniary Medical Journal
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Goats.
Mastitis.
PCR.
Traditional technology.
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| Proposed Agrovoc |
MPCR;
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| Publication Type |
Journal
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