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Effect of antioxidant enzymes supplementation during different freezing stages on semen characteristics of Friesian bulls
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Abstract: The current study was carried out at the International Livestock Management Training Center (ILMTC), Sakha belonging to the Animal Production Research Institute, Ministry of Agriculture, during the period from March to May 2007. Semen was collected by artificial vagina from five Friesian bulls, all around four years old. Semen ejaculates were collected twice weekly and held in a water bath at 35-37°C, then only ejaculates having good mass motility were pooled for each collection day for 10 weeks (100 ejaculates). On each collection day, semen was pooled and divided into 5 parts; the first was diluted with Tris-egg yolk extender without antioxidant supplementation (control), while the others were diluted with Tris-egg yolk extender supplemented with superoxide dismutase (SOD) at levels of 150 and 300 IU or catalase at levels of 200 and 400 IU. Pooled ejaculates were further processed for freezing using 0.25ml French straws. Semen diluted at a rate of 1:20 was placed into a refrigerator at 5°C for 4 h to equilibrate. At the end of equilibration period; extended semen was packaged in straws and stored at -196°C for at least one month. Thereafter, frozen semen was thawed by dipping the straws into a water bath at 38°C for 30 seconds. Percentages of motility, live sperm and intact acrosome were determined and recovery rate was calculated for post equilibrated and post-thawed semen.
Results revealed that there were no significant differences of various sperm parameters (percentages of motility, live sperm and intact acrosomes) in post-diluted semen. The highest percentage of motility (66.5%) and intact acrosomes (72.4%) were obtained with 400 IU of catalase, while the highest live sperm percent (70.1%) was recorded with 300 IU SOD. The corresponding values of recovery rates during equilibration period were 94.4, 96.9 and 98.3%, respectively. Catalase at a rate of 400 lU showed the best percentages of sperm motility (51.5%).
live sperm (53.8%) and intact acrosomes (59.4%), while level of 150 IU SOD showed the lowest improvement as compared to control. The highest recovery rate of sperm motility (77.4%) and intact acrosomes (82.1%) after freezing were significantly (p<0.05) higher for 400 IU of catalase. while the highest recovery rate of live sperm was obtained with 300 IU SOD (76.0%). Such trend supported the beneficial effects of catalase on motility and acrosome status and SOD on live sperm during equilibration and freezing.
In conclusion, supplementation of Tris-egg yolk extender, used for dilution of bull semen during freeze processing, with 400 IU of calalase or 300 IU of superaxide dismalase improved percentages and recovery roles of motility live sperm and intact acrosomes in post-thawed semen.
Keywords: Bull, catalase, superoxide dismutase, equilibration, freezing.
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| Publication year |
2009
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| Availability location |
معهد بحوث الانتاج الحيوانى - شارع نادى الصيد- الدقى - الجيزة
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| Availability number |
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| Organization Name |
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| City |
الغردقة
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| serial title |
Egyptian Society. Animal Redproduction Fertility Twenty First Annual congress
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| Author(s) from ARC |
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| External authors (outside ARC) |
شريف عبد الونيس جبر
جامعة طنطا
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Antioxidants.
Bulls.
Catalase.
Enzymes.
Freezing.
Semen.
Superoxide dismutase.
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| Proposed Agrovoc |
equilibration;
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| Publication Type |
Conference/Workshop
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