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Genetic diversity assessment of two chickens native breeds using DNA molecular markers
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Abstract: The aims of the current study were to assess genetic diversity, conduct genetic structure, and evaluate usefulness of an individual assignment test for two chicken breeds; Fayoumi and Dandrawi, using six microsatellite markers. A total of 76 distinct alleles were observed across the two breeds, and 14 of the 76 alleles (18.42%) were unique. Mean observed heterozygosity for a breed was 0.22 and 0.38 for Fayoumi and Dandrawi, respectively. Significant deviations from the Hardy-Weinberg equilibrium were observed at all locus-breeds combinations showing excess of heterozygotes in many cases. Assignment test could correctly allocate individuals at the breed level to their origins, with a high accuracy (97.3%). Individual-based genetic characterization would be a usable step to conserve chicken genetic resources. Here, guidelines for future breeding and management of these two breeds by the poultry industry are provided. The results obtained here conclude a high variability of Dandrawi breed and directs a more attention conducted such breed. In addition, the markers used herein confirmed their utility in assessing and differentiate among genetic background of different breeds. INTRODUCTION An assessment of the genetic structure utilizing mitochondrial DNA (Dong et al., 2002), random amplified polymorphic DNA (RAPD) (Smith et al., 1996 and Singh and Sharma, 2002), DNA fingerprinting (Siegel et al., 1992) and microsatellites (Kaiser et al., 2000 and Takahashi et al., 1998) were widely used to study genetic variability among and within populations. Currently, microsatellites have been widely applied in the genetic appraisal, since they are abundant, randomly distributed in the genome, highly polymorphic, and show co-dominant inheritance (Groenen et al., 2000 and Crooijmans et al., 1993). Recent information in literature have revealed that microsatellite markers are more accurate and efficient method for estimating genetic variation than other methods that have been used previously (Chen et al., 2004 and Takezaki and Nei, 1996). However, only a limited number of investigations have used microsatellites across Egyptian indigenous chicken populations (Roushdy et al., 2008b). Assignment test could correctly allocate individuals at the line level to their origins, with a high accuracy. Furthermore, individual-based genetic characterization would be a usable step to conserve chicken genetic resources. Here, guidelines for future breeding and management of these lines by the poultry industry are provided (Tadano et al., 2007). The current study was carried out to estimate genetic diversity of two indigenous chicken breeds in Egypt and to evaluate the genetic variability, based on 6 microsatellite markers together with many individuals per breed assayed. At the same time, availability of an individual assignment test using Arlequin 3.11 package for chicken populations was demonstrated.
The results from the current study would contribute to the appropriate managements avoiding loss of genetic variability in these breeds and to future improvements for these breeds.
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| Publication year |
2009
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| Pages |
231-242
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
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| Availability number |
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| Organization Name |
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| City |
طابا
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| serial title |
5th International Poultry Conference
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| Author(s) from ARC |
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| External authors (outside ARC) |
طارق محمد عبد القادر طنطاوى
جامعة عين شمس
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| Agris Categories |
Animal husbandry
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AGROVOC
TERMS |
Chickens.
DNA.
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| Proposed Agrovoc |
Genetic diversity assessment;genetic structure;
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| Publication Type |
Conference/Workshop
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