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In vitro maturation of camel oocytes as affected by different media during breeding and non-breeding seasons
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Abstract: A total number of220 clinically healthy she-camel was used in this study. The age of these camels varied from 5 to 10 years and their weights were approximately 500-600 kg. Two experiments were carried out. The first experiment aimed to define the effect of different seasons of the year on follicular fluid components and ovarian activity either in the right or left ovary. The second experiment designed to define the effects of various maturation media (TCM 199, Ham's F-10, Basal and Hank's) on the in vitro maturation of camel oocytes during breeding and non-breeding seasons. In the first experiment, the obtained results showed that ovary weight and number of corpora lutea were significantly (P < 0.05) higher during spring, winter and autumn seasons, than summer season. Numbers of the normal follicles were significantly (P < 0.05) higher during spring, while the atretic follicles were significantly (P < 0.05) higher during summer season than other seasons. Oocytes recovery, compact oocytes complexes (COC's) and partially denuded cumuls oocytes (PDCO) were significantly (P < 0.05) higher during autumn, while expanded cumulus oocytes (ECO) and denuded cumulus oocytes (DCO) were significantly (P < 0.05) higher during spring and winter seasons than other seasons of the year. The highest (P < 0.05) activities of follicular fluid aspartate aminotransaminase (AST), alanine - aminotransaminase (ALT), alkaline phosphatase (ALP) and acid phosphatase (ACP) enzymes were recorded during summer and the lowest (P < 0.05) activity was recorded during spring season. The highest (P < 0.05) values of follicular fluid potassium and calcium were recorded during winter and the lowest (P < 0.05) values were recorded during summer season. Testosterone concentration was significantly (P<0.05) higher, however cholesterol concentration was significantly (P < 0.05) lower during summer season, meanwhile oestradiol-17β concentration was significantly (P < 0.05) higher during winter season than other seasons of the year. Ovary weight, number of the corpora lutea (CL) and number of the normal follicles in the left were significantly (p< 0.05) higher than the right ovary, while the number of the atretic follicles in the right was significantly (P<0.05) higher than the left ovary. Oocyte recovery and oocyte status (COC's, PDCO, ECO and DCO) in the left ovary were significantly (P < 0.05) higher than the right one. In respect to ovary side, AST, ALT, ALP, ACP, sodium and testosterone concentration of follicular fluid in the left ovary were significantly (P < 0.05) lower than the right one. Cholesterol, potassium, calcium, inorganic phosphorus and oestradiol-17β concentrations in the left were significantly (P < 0.05) higher than the right ovary. In the second experiment, results revealed significantly (P<0.05) higher cumulus expansion, meiosis metaphase I (MI) and metaphase II (MII) than the non-breeding season. When the type of culture media there was no differences in cumulus expansion except with basal medium which produce the lowest incidence in both breeding and non-breeding season. In breeding season, TCM-199 medium showed the highest rate (P<0.05) of MII oocytes, while in non-breeding season, TCM-199 and Ham's F-I0 media showed the highest rates (P<0.05) of MII oocytes.
[A.E.B. Zeidan, M.A. EI-Harairy, Sh.A. Gabr, M.A. Tag EI-Dien, S. A. Abd EI-Rahman and A.M. Amer.In Vitro Maturation of Camel Oocytes As Affected By Different Media during Breeding and Non-Breeding Seasons.
Key words: Camels, season, ovary, follicular fluids, oocytes, in vitro maturation.
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| Publication year |
2011
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| Pages |
460-472
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
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| serial title |
Journal of American Science
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| ISSN |
1545-1003
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| Author(s) from ARC |
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| External authors (outside ARC) |
مصطفى الحرايرى
جامعة المنصورة
صباح عبد الحيمد عبد الرحمن
جامعة المستنصرية - العراق
احمد موسى عامر
جامعة المنصورة
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Camels.
Ova.
Ovaries.
Seasons.
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| Proposed Agrovoc |
follicular fluids;in vitro maturation;
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| Publication Type |
Journal
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