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Effect of centrifugation on viability, acrosomal status and enzymatic activity of spermatozoa and sex ratio of rabbit kits
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Abstract: Twenty bucks and two–hundred eight multiparous lactating New – Zealand White (NZW) does rabbit, were used in the present study. Semen was collected and divided into six equal portions. The first portion was kept as a control (without centrifugation). The second, third, fourth, fifth and sixth portions were centrifuged through Percoll solution at 250, 500, 750, 1000 and 1250 xg for 15 minutes, respectively. The sperm plugs were resuspended to a final concentration of 60 x 106 sperm/ml in sucrose-yolk-citrate extender. The extended semen samples were then stored at 5ºC for 3 days. After each storage time (0, 1, 2 and 3 days), semen quality and enzymatic activity were estimated. Fertility rate and sex ratio of the does artificially inseminated with the centrifuged and non- centrifuged semen, were also assessed.
The obtained results showed that the percentage of motile spermatozoa and live spermatozoa with intact acrosome increased significantly (P<0.01), while the percentages of the live detached acrosome and dead spermatozoa with intact or detached acrosomes and leakage of aspartate-aminotransferase (AST) and alanine – aminotransferase (ALT) enzymes into the extracellular medium were significantly (P<0.01) decreased with centrifuged semen as compared to the non- centrifuged one. The highest (P<0.01) value of sperm motility and spermatozoa with the live intact acrosome and the lowest (P<0.01) values of the live detached and dead spermatozoa with intact or detached acrosomes and amounts of AST and ALT enzymes were recorded with the centrifuged semen at 500 and 750 xg for 15 minutes. The percentage of sperm motility and spermatozoa with the live intact or detached acrosomes decreased significantly (P<0.01), while the percentages of dead spermatozoa with intact or detached acrosomes and amounts of AST and ALT enzymes were increased significantly (P<0.01) with the increase in of storage times at 5ºC for up to 3 days in both centrifuged and non-centrifuged rabbit semen. The fertility rates and total number of kits at birth were not significantly different in does artificially inseminated with the centrifuged than in those with the non-centrifuged semen. Centrifugation of semen at 500 and 750 xg insignificantly increased the fertility rate than at 250, 1000 and 1250 xg for 15 minutes. However, centrifugation of semen before artificial insemination increased significantly (P<0.05) the proportion of females kits and decreased significantly (P<0.05) the proportion of the male kits as compared to the non-centrifuged semen.
Key wards: Rabbit semen, centrifugation, Percoll gradient, Fertility, sex ratio.
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| Publication year |
2008
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| Pages |
114-123
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
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| serial title |
The 1st Egyptian Conference on Rabbit Science Animal Production Department
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Centrifuging.
Fertility.
Rabbits.
Semen.
Sex ratio.
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| Proposed Agrovoc |
Percoll gradient;artificially inseminated;motile spermatoz;
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| Publication Type |
Conference/Workshop
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