Effect of filtration of post-diluted semen by Sephadex on freezing ability and fertilizing capacity of buffalo semen
Abstract: This study aimed to evaluate the effect of Sephadex column filtration technique of post-diluted semen on freezing ability and fertilizing capacity of buffalo semen. Four Egyptian buffalo bulls were used for semen collection. Semen was extended (37°C) at a rate of 1:20 and filtered using Sephadex column with G-75 (T2) or G-100 (T3) as compared to un-filtrated semen (T1). Semen in all treatments was evaluated for progressive motility, livability, abnormality and intact acrosome spermatozoa in post-diluted. post-equilibrated and post-thawing semen. Pregnancy diagnosis was performed at 50 d post-insemination. Results showed that T2 and T3 improved (P<0.05) percentage of motility, livability, abnormality and intact acrosome spermatozoa in post-diluted. post-equilibrated and post-thawed semen as compared to T1, being better with T2 (G-75) than T3 (G-100). For buffalo cows inseminated with post-thawed semen. conception rate was 73.3, 63.3 and 56.7% in T2, T3 and T1, respectively (P<0.05).
The present results indicate that using Sephadex (G-75) column filter technique has beneficial effects on improving semen quality and fertilizing capacity of buffalo spermatozoa by removal the abnormal and dead spermatozoa in buffalo semen, which can have an adverse effect on their fertilizing potential.
Keywords: Buffalo semen, filtration, Sephadex, sperm characteristics, fertility.
Publication year 2013
Pages 647-657
Availability location معهد بحوث الانتاج الحيوانى-شار ع نادى الصيد - الدقى- الجيزة
Availability number
Organization Name
City المنصورة
serial title Journal Animal and Poultry Production, Mansoura University
ISSN 2090-3642
Author(s) from ARC
External authors (outside ARC)
    محمد السيد الشعراوى جامعة كفر الشيخ
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Artificial insemination. Biological preservation. Fertility. Freezing. Semen.
Proposed Agrovoc Buffalo bull semen;Duck egg yolk;ejaculates;
Publication Type Journal
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