Ultrastructure Study of Frozen-Thawed Spermatozoa of Friesian Bulls Treated by Certain Anthelmintic Drugs
Abstract: Effect of three anthelmintic drugs (ivenllectin, levamisole and albendazole) on the ultrastructure of spermatozoa of frozen- thawed semen Friesian bullswere used in the present study. 16 bulls were divided into four equal groups: group 1 , served as control untreated bulls (unfrozen and frozen semen); groups 2, 3 & 4 served as treated bulls with ivermectin (200 μg/kg bw); levamisole (7.5mg/kg bw) and albendazole (10mg/kg bw), respectively. The drugs used at two therapeutic doses with 8weeks interval between first and second dose. Semen samples were collected at 8th and 16th weeks and evaluated to be frozen, and then after thawing of semen samples, they were processed to separate spenllatozoa for TEM examination. Control spermatozoa (unfrozen) showed common normal architecture; head (with a flat nucleus and acrosomal cap), neck, mid and tail (principal and terminal) pieces surrounded by cell membrane, normal mitochondria, longitudinal fibers and axonemes. Frozen control semen elucidated few spermatozoa with slight detached or swollen plasma membranes surrounding normal nuclei. The treatment of bulls with either first or second dose of ivermectin showed slight swollen plasma membrane of spermatozoa, loosing acrosomes, distorted mitochondria and slight deformation of the axonemes. More deformation of spermatozoa was seen in the bulls treated with two doses of livamisole, while bulls treated with albendazole demonstrated severe damage of spermatozoa. Therefore, it could be recommended to treat bulls with ivermectin, while levamisole should be used careful. Albendazole treatment should be avoided.
Key Wards: Anthelmintics - Bulls -Frozen spermatozoa - Ultrastructure
Publication year 2013
Pages 190-199
Availability location معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة
Availability number
Organization Name
serial title Journal of American Science
ISSN 1545-1003
Author(s) from ARC
External authors (outside ARC)
    نبيلة الدسوقى كلية العلوم- جامعة طنطا
    غادة ابو طبل كلية العلوم- جامعة طنطا
    رباب كامل كلية العلوم و الفنون- جامعة جازان- المملكة العربية السعودية
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 Anthelmintics. Benzimidazoles. Bulls. Drugs. Ultrastructure.
Proposed Agrovoc Frozen spermatozoa;ivenllectin;thawed semen;
Publication Type Journal
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