Effect of low density lipoproteins in extender on freezability and fertility of Egyptian buffalo bull semen
Abstract: Semen from three Egyptian buffalo bulls was collected once weekly and ejaculates with more 75% progressive motility and more 85 % normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bulls effect. Seven extenders were used: Tris 20 % egg yolk extender with 7 ml glycerol as a control (T1), and substitution of whole egg yolk with 4, 6, 8, 10, 12 and 15 % low density lipoprotein (LDL), T2-T6, respectively. Semen was diluted to 80 xl06 sperm/ml, packaged into 0.25 ml straws, cooled, held at 5ºC for 4h, and then Forzen in liquid nitrogen (LN) and stored at -196ºC for at least one month. Sperm progressive motility, intact acrosome Gild plasma membrane integrity were assesd at post dilution, equilibration, post-thawing (at 37ºC for 30 sec.) and after 30 days storage in LN. This study reveled that LDL extenders were more effective in preservation of progressive motility, intact acrosome and integrity of the plasma membrane of buffalo spermatozoa than whole egg yolk extender sperm progressive, intact acrosome and plasma membrane integrity were much higher (P < 0.05) in the 12% LDL extender (63.3, 77.17 and 71.3% respectively) vs. 35, 40.8 and 34. 7% in the control 20% EY extender at post-thawing process, respectively. Fertility rates were higher in extender containing 12% LDLs compared with the control (72.7% vs. 50%, respectively). It was concluded that LDL (12%) in extender improved the freezability and fertility of buffalo spermatozoa.
Key words; Buffalo Bull Semen; LDL; Freezability; Fertility
Publication year 2012
Availability location معهد بحوث الامتاج الحيواني- شارع نادي الصيد- دقي- جيزة
Availability number
Organization Name
serial title Scientific Papers, Animal science, Series D
ISSN 2285-5750
Author(s) from ARC
External authors (outside ARC)
    محمد السيد الشعرواى زراعة كفر الشيخ
    ابراهيم سعد الشماع زراعة كفر الشيخ
    مصطفى عبد الرحمن زراعة كفر الشيخ
    ابراهيم عبد الرازق زراعة كفر الشيخ
Agris Categories Animal physiology - Reproduction
AGROVOC
TERMS		 African buffaloes. Bulls. Fertility. Semen. Semen preservation.
Proposed Agrovoc LDL;
Publication Type Journal
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