: effect of dimethyl diphenyl bicarboxylate ,DDB on some immunological and histopathological parameters in rabbits vaccinated with pasteurella multocida vaccine

Abstract: Twenty apparently healthy Newzealand white male rabbits (average weight = 818.3 + 12.8 grams) were divided into four groups (five animals each), the first group was served as the normal control, the second group was orally administered with 2mg Dimethyl Diphenyl Bicarboxylate (DDB)/kg.b.w. for 28 successive days, the third group was twicely vaccinated (two weeks apart) with pasteurella multocida (sero var 5: A) vaccine, and the fourth group was vaccinated also (as in the third group) along with DDB treatment (as in the second group). At the 29th, day (21 days post second vaccination) the blood, serum, spleen and liver samples were obtained from all animals. The haematological, serological, serum protein electrophoretical, biochemical and histopathological studies were carried out. Results indicated that DDB treatment induced immunostimulant activity due to the significant increase in the total immunoglobulins (Gamma – globulins), lymphocyte percentage and prominent lymphocytic aggregation within the lymphoid follicles of the spleen, but the increase in the indirect Haemagglutination specific antibody titer by DDB treatment was non-significantly against pasteurella multocida vaccine, also the DDB induced significant increase in red blood corpuscles and haemoglobin concentration and reduced ALT-enzyme activity with the improvement in liver histology in vaccinated rabbits compared to the only vaccinated animals. Based on our study, it should be considered that DDB as a promising immunostimulant, blood tonic and hepatoprotective drug.

Publication year

2004

Pages

49-63

Availability location

مكتبة معهد بحوث صحة الحيوان

Availability number

Organization Name

Animal Health Research Institute (AHRI)

City

المنصورة

serial title