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comparison of pcr amplification of the fim a gene and cultural method for detection of salmonella in feed and water
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مقارنة اختبار بى سى ار باستخدام الفيم A جين وطريقة العزل البكتيرى فى اكتشاف السالمونيلا فى الاعلاف والمياه
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Abstract: Bacteriological examination of local and imported feedstuff samples revealed that 11 out of 600 feedstuff samples harboured salmonellae with an incidence of 1.8% the highest incidence of isolation was in imported bone and meat meal (4%) .A polymerase chain reaction was performed by amplification fim A gene as specific method for detection of salmonella in feed and water In order to obtain higher specificity , we selected a series of primers internal to the fim A gene sequence . the concordance percent between conventional cultural method and PCR of feed and water were 86.6 % and 100% respectively.this assay enable to detect 10 C.F.U./ 100 mi water and 10 C.F.U /25 .this rapid assay provides a sensitive and specific means of screening drinking water samples as well as geedstuff samples for the presence of salmoella spp
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| Publication year |
2002
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| Pages |
627-638
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| Availability location |
مكتبة معهد بحوث صحة الحيوان
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| Availability number |
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| Organization Name |
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| City |
القاهرة
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| serial title |
المؤتمر العلمى السابع - كلية الطب البيطرى جامعة القاهرة
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| Author(s) from ARC |
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| Agris Categories |
Veterinary science and hygiene - General aspects
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AGROVOC
TERMS |
Cultural methods.
Feed additives.
PCR.
Salmonella.
Water.
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| Proposed Agrovoc |
ampliication;fim a gene;
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| Publication Type |
Conference/Workshop
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