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Effect of addition different glutamine levels in lowdensity lipoproteins (12% LDL) extender on freezability and fertility of Egyptian buffalo bulls semen
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Abstract: The objectives of the current study were to assess the efficacy of addition of different glutamine levels diluted in low density lipoproteins (12% LDL) on the freezability and fertility of Egyptian buffalo semen. Therefore, semen was collected by artificial vagina from three buffalo bulls, around 34 years old, once weekly and held in a water bath at 35-37°C, then only ejaculates having more than 70% advanced motility were pooled for each collection day (for 4 consecutive weeks). On each collection day, pooled semen was diluted with basic 12% LDL extender and divided into 7 parts; the first one was served as control without any glutamine supplementation, while the others, glutamine (Gln) was added at levels of 10, 20, 30, 40, 50 and 60 mM. Semen diluted at a rate of 1:20 was placed into a refrigerator at 5°C for 4 b to equilibrate. At the end of equilibration period, extended semen was packaged in 0.25 ml French straws .and stored at -196°C for at least one month. Thereafter, frozen semen was thawed by dipping the straws into a water bath at 37°C for 30 seconds. Percentages of advanced motility, live, intact acrosome and plasma membrane integrity of buffalo spermatozoa were determined and recovery rate was calculated for post equilibrated and post-thawed semen. The results indicated that low Glutamine concentrations (10, 20 and 30 mM) improved significantly (P < 0.05) semen motility, live, intact acrosome and plasma membrane integrity than 50 mM or 60 mM glutamine and non significant with 40 mM after 30 days of storage in LN. In addition 12% LDL extender with 10, 20 and 30 mM glutamine resulted in the highest conception rates (72.2, 73.3 and 66.6%, respectively) followed with 40 mM Gin (62.5%), 50 mM Gln (55.6%), and 60 mM Gln (50.0%).
In conclusion, diluents based on 12% LDL plus 10 and 20 mM lead to improvement of frozen semen characteristics and fertility of Egyptian buffalo bulls semen Keywords: Buflalo bulls; Cryopreservation; Semen characteristics; 12% LDL extender; Glutamine.
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| Publication year |
2013
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| Pages |
9-17
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
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| serial title |
The 4th Scientific Conference of Animal Production Research Institute (APRI)
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| Author(s) from ARC |
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| External authors (outside ARC) |
ابراهيم سعد الشماع
زراعة كفر الشيخ جامعة طنطا
مصطفى عبد الرحمن ابراهيم
زراعة كفر الشيخ- جامعة طنطا
محمد السيد الشعراوى
زراعة كفر الشيخ- جامعةطنطا
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Biological preservation.
Fertility.
Freezing.
Glutamine.
Semen.
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| Proposed Agrovoc |
12% LDL extender;Semen characteristics;Buflalo bulls;
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| Publication Type |
Conference/Workshop
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