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Impact of nano?zinc?oxide as an alternative source of zinc in date palm
culture media
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Abstract: Nanotechnology is a rapidly spreading field to be applied in agriculture. From all kinds of nutrients, zinc nanoparticles
had a great interest in investigations due to its importance for plant as it involved in many growth processes. Presently, rare
literature is available about nanoparticles effects on date palm in vitro propagation, especially zinc oxide nanoparticles (ZnONPs).
Therefore, ZnO-NPs were used in this investigation to study their effect on different stages of date palm (cv. Khalas)
somatic embryo protocol. Instead of zinc sulfate (
ZnSo4) in Murashige and Skooge medium (8.9 mg/l), lower concentrations
of ZnO-NPs (0.2, 0.1, 0.05 and 0.025 mg/l) were added. Generally, results showed that ZnO-NPs accelerated superior values
compared with ZnSo4,
as a control treatment, at all stages of date palm tissue culture protocol. Lower concentrations of
ZnO-NPs (0.025 and 0.05 mg/l) gave better values compared with higher concentrations (0.1 and 0.2 mg/l) in callus growth,
globularization, embryo formation number, secondary embryo number and shoot length. Whereas, the highest concentration
showed higher shoot number and rooted plantlet length. Antioxidants enzymes and ROS and their relations were identified
in date palm leaflets. As we know it is the first investigation of ZnO-NPs and their morphological and biochemical effects
on date palm in vitro culture.
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| Publication year |
2022
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| Organization Name |
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| Author(s) from ARC |
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| Publication Type |
Journal
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